EPPO-Q-bank

A database to support plant pest diagnostic activities

Curator Insects database

Dr Tibor Bukovinszki
National Reference Centre, Netherlands Food and Consumer Product Safety Authority (NVWA)
the Netherlands

Curator Insects database

Jean-Claude Streito
INRA-CBGP Montferrier-sur-Lez
France

Curator Insects database

Eric Pierre
INRA-CBGP Montferrier-sur-Lez
France

EPPO-Q-bank Arthropod database

Qbank's Arthropods database was first designed and developed as part of the QBOL project by J.-Y. Rasplus (INRAE-CBGP, France), A. Cruaud (INRAE-CBGP, France), A.J.M. Loomans (NVWA, the Netherlands) and collaborators between 2011 and 2015. Currently, the EPPO-Q-bank Arthropod database contains DNA sequences (barcodes) of quarantine arthropod species for Europe and their closest relatives. The choice was made to sequence the mitochondrial Cytochrome c oxydase I standard barcode fragment (COI) as well as the nuclear internal transcribed spacer 2 (ITS2) to provide a reliable identification tool for all developmental stages of those Q-arthropods and congeneric pest species.

Most species sequenced originate from Palearctic and Nearctic regions. 

To facilitate, identification of quarantine species about 100 closely related species have been sequenced (including also some pests).

A list of references used for the development of the Arthropods database can be consulted.

The distribution as well as relevant EPPO-protocols for Arthropods can be downloaded from the EPPO Global Database https://gd.eppo.int/


Warning regarding the identification of quarantine Arthropods using barcoding.


This molecular database supports reliable identification of most quarantine species of Arthropods included, mostly using a 658-base pair fragment in the gene encoding the mitochondrial cytochrome c oxidase 1 (COI), corresponding the standard barcode as defined by Hebert et al. (2003)*.

However, several genera and complex species cannot be reliably identified using this gene. It is essential before providing a BLAST result, to carefully verify that the taxon does not belong to a listed taxonomic group for which the barcode is not working.

The database also contains a few sequences of ITS2 gene, their use in practice is limited however as very few ITS2 sequences are available in international databases.

A list of taxa included in the database and a list of taxa for which the greatest concern is required are provided below.


Content of the database

Although EPPO-Q-bank is the most complete database for quarantine arthropods for Europe, several species are still missing. Thus, it is recommended to consult the list of taxa included to verify that the targeted species is included.

When the BLAST query does not match or when the best similarity rate is less than 98%, the identification should be considered as undetermined or uncertain, but not negative.


List of taxa included in the molecular database whose identification is uncertain with the standard COI barcode. 






Order

Family

Interspecific discrimination between closely related species inconclusive

Intraspecific discrimination between subspecies or biotypes     inconclusive

Coleoptera

Chrysomelidae


Diabrotica sub spp.

Coleoptera

Cerambycidae

Monochamus spp.


Coleoptera

Cerambycidae

Trichoferus spp.


Lepidoptera

Tortricidae 

Tortricidae: several species are very close together 

Torticidae: not enough specimens in database to reflect intraspecific diversity

Lepidoptera

Tortricidae

Choristoneura fumiferana / pinus


Lepidoptera

Lasiocampidae

Dendrolimus spp.


Hymenoptera

Pamphiliidae

Cephalcia spp. 


Hymenoptera

Tenthredinidae

Pristiphora spp. 


Diptera

Tephritidae

Rhagoletis completa / zoqui / ramosae 



Tephritidae

Rhagoletis pomonella/mendax



Tephritidae

Anastrepha fraterculus / obliqua / suspensa 



  Tephritidae

  Ceratitis fasciventris / anonae / rosa / quilicii


Tephritidae

Bactrocera dorsalis / carambolae


Hemiptera

Aleyrodidae

Aleurocanthus spp.

Bemisia tabaci biotypes

Hemiptera

Miridae

Lygus spp.




* HEBERT P. D. N., CYWINSKA A., BALL S. L. & DEWAARD J. R., 2003. – Biological identifications through DNA barcodes. Proceedings of the Royal Society of London B, 270: 313-321.