A database to support plant pest diagnostic activities

Curator Nematodes database

Ir Evelyn van Heese
Phytosanitary and taxonomic expertise and collections
Netherlands Institute for Vectors, Invasive plants and Plant health (NIVIP)
the Netherlands

Curator Nematodes database

Dr. Sebastian Kiewnick
Taxonomic expertise and collections
Julius Kühn-Institut
Federal Research Centre for Cultivated Plants
Institute for Plant Protection in Field Crops and Grassland, Braunschweig

EPPO-Q-bank Nematodes database

The EPPO-Q-bank Nematodes database contains DNA barcodes (sequences) of quarantine nematodes and their close relatives. Currently, this database focuses on nematode species that are regulated in the European Union and are listed as A1/A2 organisms by the European Plant Protection Organization (EPPO).

The EPPO-Q-bank Nematodes database comprises sequence data from specimen of properly documented type material or reference populations of quarantine plant parasitic nematodes species and their close relatives. This information is in support of an accurate identification of nematodes by plant protection organizations, inspection bodies and private laboratories.

The main focus of EPPO-Q-bank Nematodes is on European quarantine nematodes and other economically important plant-parasitic nematodes. For accurate distinction of regulated from non-regulated nematode species sequence data of the latter are important. For nematode species which are listed as A1/A2 organism, relevant EPPO documents such as diagnostic standard or distribution maps, can be downloaded from GLobal Database https://gd.eppo.int/

The EPPO Q-bank database is continuously updated by a team of curators with connections to relevant  physical, phytosanitary collections (e.g. FERA, JKI and PPS Wageningen). Following a thorough quality control, sequences obtained from specimen belonging to reference populations or type material are deposited in the database. Using a single BLAST or multilocus search, sequences generated by using the PM 7/129 barcoding standard protocol can be used for identification of an unknown nematode species. In addition, information on the availability of supplemental material such as permanent slides in collections or living material from reference populations is provided. Information on the availability in collections can be obtained in the specific specimen page.

How to use barcoding for identification of quarantine nematodes?
Detailed protocols for the DNA isolation and PCR amplification of the genera are included on this website, as well as a flow diagram showing the workflow for the identification of specific quarantine species included in the database.

The information in this database was compiled by the EPPO-Q-bank curators to the best of their knowledge. If you have questions or you want to contribute data to the database please contact us.

The distribution as well as relevant EPPO-protocols for nematodes can be downloaded from the EPPO Global Database https://gd.eppo.int/

Here you can find selected links.