Minimum requirements for submission of virus isolates to the EPPO-Q-bank Plant Viruses and Viroids database and collections
All isolates included in EPPO-Q-bank should be publicly available from a collection under a quality management system, preferably including a back-up at another location. The identification is done by the depositor and/or by the Q-bank curators. Isolate authentication is pursued prior to making virus isolates available via EPPO-Q-bank.
Information on isolates
- Original host plant species*
- Propagation host (if not original host)
- Date of collection*
- Country where original host plant was found*
- Name of institute that collected or identified (characterised) the isolate*
* For isolates already present in collections, this information and/or data might not be available. For these 'old' isolates the marked information and/or data are desirable. For 'new' isolates they are considered minimum requirements. Note that not all these data are included in the database.
Information on isolate (for database)
- Identity of the isolate, preferably based on two different properties
- Consensus sequence, based on ‘high quality’ sequence data
- PCR primers (names and sequences) and/or protocol (e.g. NGS) used (if applicable)
- Optional: reference to publication, accession number NCBI GenBank
Information on isolate (for collection)
- Propagation host
- Verification of identity (indicate methods used: electron microscopy, serological test, specific molecular test, sequence analysis, symptomatology on test plants)
- Verification of viability on test plants
- Verification of purity (as far as possible) by electron microscopy or other relevant techniques
- Location of the material (from where the isolate can be obtained; contact details)
For viruses and viroids barcodes are not applicable since they lack universal 'household' genes. In fact, the whole genome should be considered as a barcode. At present, it is not realistic to ask for whole genome sequences for each isolate to enter Q-bank. However, complete genome sequences are appreciated if available.
Once data are added into the database, the name of the institute(s) depositing or contributing will be credited on the EPPO-Q-bank website.