Soybean mosaic virus(SMV000)
Isolate
- Name: SMV_2020_002
| General information - specimen description | |
| References | Van Bentum S. et al. (2022) First report of soybean mosaic virus on soybean in The Netherlands. Plant Disease 106 |
| Sampling - Origin | |
| Country of sampling | Netherlands |
| Sampling - Substrate from which the specimen / isolate / strain was isolated or collected | |
| Matrix/Substrate type | plant |
| Scientific name of the plant or vector | Glycine max |
| Additional information on substrate | Infected leaf material Soybean cv. Green Shell |
| Sampling - When | |
| Sampling/isolation date | 2020-7 |
| Sampling - Who | |
| Collector name | S. van Bentum |
| Identification - General | |
| Identification method | RT-PCR and High Throughput Sequencing (Illumina) |
| Identification - Remarks | |
| Remarks on molecular methods | Initial identification by Sanger seq on RT-PCR product generated by primmer set P9502/ CPUP (vdVlugt et al, 1999) and SMV-specific primers SMV-dT (5’-TTTTTTTTTTTTTTTAGGACAAC-3’) and SMV-Nib-Fw (5’- CAAGGATGARTTTAAGGAG-3’) |
| Remarks on test-plant reactions (description of systemic symptoms) | Veinal chlorosis (12DPI) developing into veinal necrosis on soybean plants (unknown cv.) |
| General information on the collection | |
| Collection name | Wageningen University & Research |
| Collection information specific to the specimen | |
| Number in collection | SMV_2020_002 |
| Isolate synonyms (history) | Green Shell |
| Date of deposit in collection | 2020-11 |
| Type of material in collection | frozen material |
| Deposited by (name) | R.A.A. van der Vlugt |
| Nagoya protocol | No |
| Title | Locus | |
|---|---|---|
| SMV_2020-002 Green Shell | Complete Genome |