A database to support plant pest diagnostic activities

The barcoding protocol within Ralstonia

For identification of the Ralstonia species and the R. solanacearum phylotypes, a partial sequence of the Egl gene of approximately 840 bp is amplified using Egl primers (Wicker et al., 2007) with a M13-tag and sequenced using M13 primers.

The protocol for the conventional PCR egl for the Ralstonia solanacearum species complex identification is available in the annex 2, section 2.4 of PM 7/129.