The barcoding protocol within Xylella
After 16S rDNA-based identification of the Xylella bacterial genus according to PM7/129, the mutS barcode can be used to identify Xylella fastidiosa subspecies fastidiosa, multiplex, pauca, sandyi and morus (see also the molecular decision scheme). Please note that strains exist that cannot be unambiguously assigned to a subspecies on the basis of whole genome sequences.
The protocol for the conventional PCR 16S rDNA for bacteria is available in the annex 2, section 2.2 of PM 7/129.
The protocol for mutS for the identification of Xylella fastidiosa subspecies can be found here.
The general guidelines described in PM7/129 (2) "DNA barcoding as an identification tool for a number of regulated pests" apply.
See also PM7/024 (4) for identification of Xylella fastidiosa at subspecies level. The last updated version of this standard is available in EPPO Global database.